The Global DNA Methylation ELISA Kit from Cell Biolabs is a highly sensitive competitive ELISA for 5’-methyl-2’-deoxycytidine (5MedCyd) quantification. Validated for use directly with urine or with extracted cell or tissue DNA samples, this kit can rapidly detect and quantify 5MedCyd from a range of species.
Benefits of using the Global DNA Methylation ELISA Kit
• 50 nM 5MedCyd detection sensitivity
• Non labour-intensive procedure
• Compatible with a wide range of species
How it works
The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRPconjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.
5MedCyd ELISA standard curve (left) and 5MedCyd levels in various dilutions of human urine (right).
Material available for download
Global DNA Methylation ELISA Kit manual
Global DNA Methylation ELISA Kit citation list