Product Category: Oxidative Stress

OxiSelect Protein Carbonyl Assay Kits

Cell Biolabs OxiSelectTM Protein Carbonyl Assay Kits
The most common products of protein oxidation in biological samples are the carbonyl derivatives of Pro, Arg, Lys and Thr residues. Such derivatives are chemically stable and serve as markers for oxidative stress in most types of reactive oxygen species.

Cell Biolabs’ OxiSelect™ Protein Carbonyl Assay Kits provide rapid, efficient methods for detection of protein carbonyls. Three assay formats are available: immunoblot, ELISA or spectrophotometer. All formats are suitable for use with purified protein, plasma, serum, or cell lysate samples.

Protein Carbonyl ELISA Kit
• Sensitive: Detects samples as low as 10 μg/ml
• Greater Sample Retention: No concentration/TCA precipitation steps

Protein Carbonyl Immunoblot Kit
• No Molecular Weight Shift: DNPH Derivatization after immunoblotting allows direct comparison of oxidized and non-oxidized protein fingerprint

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8-Isoprostane ELISA Kit from Cayman Chemical

Isoprostanes are a family of non-enzymatic origin that appear as artifacts in tissues and plasma samples that have undergone incorrect storage. 8-isoprostane (8-epi PGF_2α) has been proposed as a marker of oxidative stress. The competitive ELISA kit from Cayman Chemical has been validated on numerous sample types including urine and plasma.

• Limit of detection 2.7 pg/mL (80% B/B₀).
• Sensitivity 10pg/mL (50% B/B_O).
• Flexible strip plate format.
• 96 and 480 well formats available.
• AChE tracer used
• 8-Isoprostane affinity columns available. 

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Nitrotyrosine Assay

Nitrotyrosine has been identified as a marker of inflammation . Hycult Biotech's sensitive and reproducible sandwich ELISA kit (HK501) for the detection of nitrotyrosine is sensitive to 2nM.

• Complete: Ready to use ELISA kit 
• Flexible: Two plates with twelve 8 well strips
• Sensitive: Working range of 2- 1,500nM
• Value for money

Oxidative Stress Biomarkers

Cell Biolabs Oxidative Stress Products

Cell Biolabs have developed a broad portfolio of sensitive, easy-to-use assays to quantify oxidative damage. Their assays use the most ubiquitous, well-published markers for each of the given molecules and can be performed using either a standard microplate reader or Western blotting.

click here for further information on assays for :

• DNA/RNA Damage
• Lipid Peroxidation
• Protein Oxidation
• ROS and Antioxidant assays

Reactive Oxygen Species (ROS) Kits from Cayman Chemical

ROS commonly refers to superoxide anions, hydrogen peroxide, hypohalous acid and hydroxyl radicals and may lead to the oxidative damage of virtually any molecule. Under normal conditions ROS are cleared from the cell by catalase, glutathione peroxidase (GSH), or superoxide dimutase (SOD). The SOD assay from Cayman Chemical accurately quantifies all three types of SOD (Cu/Zn^-, Mn^-, and Fe^-SOD).  

OxiSelect Comet Assays

The comet assay is a well-published method for measuring cellular DNA damage.

Cell Biolab's new OxiSelect™ Comet Assay Kits provide a fast, convenient way to screen for general DNA damage, regardless of the source or nature of the damage.

• Useful screening tool for various types of DNA damage
• Slides are specially treated for adhesion of low-melting agarose
• Easy visualization by epifluorescence microscopy

Myeloperoxidase ELISAs from Hycult Biotech

MPO is an important enzyme used during phagocytic lysis of engulfed foreign particles. The Hycult Biotech MPO ELISA Kit is a ready-to-use set of essential components needed to measure MPO activity safely and rapidly. The Human MPO ELISA kit is fully validated on saliva samples non invasive.

• Quantification of MPO in plasma
• Quantification of MPO during in vitro release from neutrophils
•  Ready to use
• Available against Human, Mouse and Rat forms of the target
• Strip microplate format

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OxiSelect Oxidative DNA Damage ELISA Kit

Cell Biolabs’ Oxidative DNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid quantitation of 8-OHdG in urine, serum, or other cell or tissue DNA samples.

The quantity of 8-OHdG in unknown samples is determined by comparing its absorbance with that of a known 8-OHdG standard curve.