CRISPR/Cas9 Genome EngineeringCambridge Bioscience offers a portfolio of all-in-one CRISPR-Cas9 cloning vectors with different promoter options. The CRISPR/Cas9 system uses a nuclease, CRISPR-associated Cas9 that complexes with small RNAs as guides (gRNAs) to cleave DNA in a sequence-specific manner upstream of the protospacer adaptor motif (PAM) in any genomic location. The system is comprised of separate guide RNAs (crRNA and tracrRNA) combined into a single RNA to enable site-specific mammalian genome cutting through the design of a short guide RNA. Each cloning vector expresses a human codon-optimised Cas9 nuclease as well as a gRNA sequence linked to the tracrRNA.
Benefits Of Using CRISPR-Cas9
• Target & modify any genomic sequence
• High target efficiency
• Easy to implement
Products & Services Offered
Through our partnership with System Biosciences, we offer access to a comprehensive CRISPR/Cas9 genome engineering service. To find out more, please Click Here.
We also offer pre-made knock-out CRISPR/Cas9 kits specific for each gene locus, through our partnership with OriGene Technologies. To find out more, please Click Here.
CRISPR/Cas9 Transfection Reagents
We offer a portfolio of highly effective transfection reagents for your CRISPR/Cas9 needs. To find out more, Click Here.
Endura™ Electrocompetent Cells for CRISPR/Cas9
We also offer Endura™ electrcompetent cells for reliable and efficient cloning and maintenance of CRISPR clones. To find out more, Click Here.