Large Insert Library Construction
Cambridge Bioscience offers access to a library construction service for difficult-to-clone genomic DNAs, large cDNAs and long PCR products from Lucigen. Using the first-of-its-kind BigEasy® pJAZZ® linear cloning vectors offering exceptional stability for large insert library construction, bSMART large insert libraries are delivered as frozen clones in 96- or 384-well plates with a complete report and guidelines on storing and working with the bSMART Library.
Benefits of using the BigEasy® pJAZZ® Linear Cloning Vectors
• Exceptional stability
• Enables the construction of accurate and complete libraries, from <1 kb to over 30 k
•Create libraries from A/T-rich or G/C-rich genomes
•Clone gene clusters or operons
•Inducible copy number
bSMART large insert libraries are delivered as frozen clones in 96- or 384-well plates with a complete report and guidelines on storing and working with the bSMART Library.
“TIGR had tried several times [to make a large-insert Tetrahymena library], but the largest size range we could obtain was 4-6 kb. Lucigen was able to provide a library in the pJAZZ® vector with an average insert size of 12 kb and some inserts as large as 20 kb.”
Dr. Robert Coyne, The Institute for Genome Research (TIGR)
Contact Our Specialist
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