Supplier information: Imgenex

Imgenex develops and commercializes novel reagents for the study of human biology and disease and for the production of new diagnostic assays and potential therapies for such diseases.

Their novel reagents include antibodies, gene and protein expression systems. Areas of biological interest at Imgenex include cancer, apoptosis, molecular signaling pathways, cellular aging, and metabolic and infectious diseases.

At least 11 TLR's have been identified in humans and 13 in mice. Cambridge Bioscience supplies Abs, flow cytometry validated sampler kits, TLR ligands and TLR agonists to allow you to research the full spectrum of TLR's.

• Antibodies for TLR1 to TLR13
• Abs Validated for flow cytometry, IHC (paraffin), IHC (frozen)
• Antibody sampler kits for flow cytometry, (TLR1-9)
• CpG-DNA's for TLR9 research

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Imgenex now offers two kits enabling researchers to quickly fix and prepare cells for cell surface or intracellular staining and analysis.

Proteins are expressed on either the cell surface, intracellularly, or both. Flow cytometry detection methods are typically carried out based on where the protein is thought to be localized.

Benefits

• Simple - optimised protocols for fast results
• Complete - contains necessary reagents to fix and/or permeabilize cells 
• Flexible - suitable for both conjugated and unconjugated, mono- and polyclonal antibodies
• Cost Effective - sufficient reagents for hundreds of tests

Imgenex have a growing portfolio of antibodies and inhibitors to proteins and processes involved in apoptosis signaling encompasses a wide variety of techniques including flow cytometry, western blot analysis, immunoprecipitation, immunohistochemistry, immunofluorescence micro-scopy, and blocking apoptosis. Additionally, Imgenex have recently launched RNA interference (RNAi/siRNA) tools to knock down genes involved in apoptosis. These siRNAs can be used to transiently silence a specific gene in cell lines or to generate stable knockdown cell lines.

To request your copy of the Imgenex Apoptosis Research Tools Brochure, please click here

The cell lines are ideal in the study of innate immune pathway activation and as positive controls for TLR phenotyping.

• Validated for expression by western blot and flow cytometry
• Functional validation carried out via ligand response assays
• Optimised format for immediate use

Imgenex have a wide range of agonist molecules for use with the cell lines in their TLR System range and NFkB activation levels can be detected by their NFkB SEAPorter Assay.