Supplier information: OriGene

OriGene has commercialized an extensive genebank of authentic full-length cDNA clones in a standard expression vector. In combination with related gene expression reagents, their products enable high-throughput functional gene analysis and the insights into disease processes.

*New* Clone Connector
Connecting You To The Right Clone

At Cambridge Bioscience we do not believe that you should spend time searching for your cDNA clone, ORF or shRNA construct. That is why we are pleased to offer our customers our new free to use Clone Connector Service. All you need to do is fill in our simple Clone Connector Form and our clone specialists will do the searching for you providing you with a list of suggested products that match your specifications.

To use the Clone Connector Service or to find out more, please Click Here

TrueMAB™
High Performance Monoclonal Antibodies

TrueMAB™ antibodies are high performance monoclonal antibodies providing high sensitivity and specificity for the recognition of native protein conformational structures and epitopes. Unlike other antibodies, the majority of the recombinant human proteins used as antigens are full length proteins expressed in human cells. Please click here to find out more

Introductory Offer
In recognition of these high performance products, we are pleased to offer a special 30% discount off newly added TrueMAB™ antibodies for a limited time. Please click here for further details.

OriGene’s TrueORF product line, consisting of 25 000 human and 12 000 mouse ORF Clones express the encoded transcript as a tagged protein with either a Myc-DKK or GFP tag, for ease of detection. Please click here for the TrueORF Application Guide.

TrueORF inserts are housed in a pCMV6-Entry vector and can be easily shuttled by a simple 'cut-and-paste' mechanism into any of the OriGene Precision Shuttle Destination Vectors offering a range of additional tags via 60 different Destination vectors. These tags include:

HIS, Myc, HA, DDK (Flag), Myc-DKK, Myc-HIS, HA-HIS, DDK-HIS and a range of fluorescent tags including; GFP, RFP, YFP and mKATE.

The destination vectors offer choices of:

 
OriGene’s TrueORF clones are supplied as ready to use purified plasmid DNA, which saves you valuable time compared to working with bacterial stocks.

The OriGene TrueClone collection represents the most comprehensive, commercially available cDNA collection. More than 30,000 clones obtained directly from plasmid cDNA libraries, are available in expression vectors suitable for transfection into mammalian cells and for direct, in vitro expression. 

Clones start from as little as £105. Click here to use our cDNA Clone Match Service or request info on HuSH RNAi

Also available from OriGene, HuSH shRNA: The most potent 29-mer shRNA duplex for expression knock-down, covering 100% of human gene targets.

Please click here for the TrueClone application guide and here to download the price list.

HuSh 29-mer hairpin expression clones appear to enter the RNAi pathway more efficiently and result in much higher potency and specificity than shorter expressed or oligo RNAi forms. shRNA less than 30 base pairs in length evades the radar of the mammalian interferon response and initiates strong and specific gene silencing. Please click here for the HuSh 29-mer Application Guide.

The OriGene HuSh 29-mer product line contains shRNA expression vectors offering genome wide coverage for human, mouse and rat species.

• 29mers are up to 100x more efficient than 21mers
• Supplied as 4 constructs in purified plasmid
• Empty vector and scrambled sequence controls provided
• Choice of 3 retroviral vectors (untagged, GFP or RFP)
• Transient or stable transfection or retroviral infection options
• Guaranteed successful knockdown

Please click here to request pricing information or more details on HuSh 29-mer products.

Price information can be downloaded from the following links:

Human HuSh 29mer, GFP Human HuSh 29mer and RFP Human HuSh 29mer.

We now offer Marligen’s xMAP® assays, which are available for miRNA, transcriptions factors and SNP genotyping.

Measure the presence of multiple molecular biomarkers for various genetic diseases simultaneously, through fluorescently tagged microsphere beads hybridised to labelled markers of interest.

• Extremely high throughput methods:- hundreds of tests in hours
• Large range of disease state panels from prostate cancer to diabetes.
• Small sample requirements
• Flexibility provided via custom designed panel services.

Marligen supports scientists without access to the xMAP® Luminex platform via testing services for multiplex transcription factor profiling and SNP genotyping.

For more information relating to these new upcoming assays please click here.

 

Powered by OriGene's extensive collection of TrueORF human cDNA clones, OriGene now offers >5,000 full length human proteins expressed in HEK293 cells and purified with affinity column. Please see the full range of proteins here.

• The broadest offering of human proteins produced in human cells
• Optimal preservation of protein structure, post-translational modifications and functions
• >80% purity through affinity purification
• Options for large scale production available

OriGene also offers a broard selction of 5000 monoclonal and polyclonal antibodies to human proteins for various applications, including:

• >3,500 antibodies include a tube of gene specific over-expression cell lysate as a positive control
• Target important human proteins covering various pathways
• >300 TrueMAB™ antibodies are made with authentic protein immunogens to provide superior performance recognizing native epitopes
• Anti-tag antibodies for the detection of recombinant proteins
• >900 antibodies for stem cell biology

Please click here for more information.

Introducing the latest innovation from OriGene; TrueORF GOLD Expression Validated cDNA Clones. As part of OriGene’s extensive TrueORF Tagged Clone collection of 25,000 human clones, TrueORF Gold is a premium selection of 6,000 cDNA clones that have passed the ultimate tests: sequence verification and protein expression validation. 

Don’t settle for less when you can have the “Gold Standard” for cDNA Clones.

• Tested individually by Western Blot

• Sequence verified

• Transfection ready

The new ProteoScan Cancer Lysate Arrays from OriGene are high-density reverse-phase protein arrays (RPPA, or RPA) assembled from 432 cancer and normal tissue protein lysates. With protein-specific antibodies, ProteoScan Cancer Lysate Arrays enable quantitative identification and validation of protein expression profiles and post-translation modifications associated with 11 types of cancer.

• 432 tissue lysates from pathologist-verified normal and cancer specimens
• 11 cancers represented (25 cancer samples +15 normal samples per cancer type)
• Each sample in 4 dilutions in triplicates for quantitative and statistical analysis
• Extensive positive and negative controls

Please click here for more information.

In a "first of its kind" launch OriGene now offers the service to generate Mass Spectrometry (MS) standards for 5,000 human proteins. Produced in human HEK293T cells and labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, the full length proteins with appropriate post-translational modifications are the best identification and quantification standards.

Heavy isotope labeled human proteins are ideal internal standards for SRM/MRM (single reaction monitoring, multiple reaction monitoring) mass spectrometry analyses for high throughput proteomics and clinical biomarker research.

• Accurate quantification
• Identify the best SRM and MRM transitions through experimental data
• Authentic posttranslational modifications by using human HEK293T cell line.
• Higher data consistency than synthetic peptide internal standard
• Suitable for all types of MS equipment
• Over 90% incorporation efficiency

For further details please click here.

Origene's Tagged Over-expression Lysates for Western Blot applications :

• 10,000 over-expression lysates of full length human proteins
• Expressed in mammalian HEK293T cells
• C-terminal myc-DDK* tag for easy expression detection and isolation
• Including a vector only transfection lysate as negative control

Applications

• Positive controls in Western Blot
• Antibody affinity titration
• Antigens for antibody production
• Reverse phase protein arrays

Validate Biomarkers Across Hundreds of Tissues in Less Than 2 Hours

TissueScan is a unique qPCR array of first-strand cDNA from hundreds of clinical tumor samples. It provides fast and accurate gene expression profiling across a large number of cancer patient samples. High-quality cDNAs were prepared from well-documented cancer biopsy samples, normalized and assembled into ready-to-use gene expression panels. This alleviates the tedious work of sample collection and RNA/cDNA preparation, while facilitating quick and reliable profiling of gene expression levels across cancer progression stages.

TissueScan combines the high sensitivity and specificity of the RT-PCR methodology with a well-designed multi-sample format. It is an excellent tool for validation of potential cancer markers such as those obtained by microarray analysis.

Origene's GFC-Transfection Arrays are sets of CMV-driven expression ready individual cDNA clones arrayed in both 384 or 96 well formats. Each clone is from OriGene’s TrueClone collection of full-length human cDNA clones and each clone is available separately for immediate shipment. 

GFC-Transfection Arrays are standardized, easy to use and ready for assay. With this product, researchers are no longer restricted to study one gene at a time, but can functionally screen thousands of genes (up to the entire genome) in fast, inexpensive and convenient gain-of-function assays.

click here for more information on Origene's GFC Arrays