Supplier information: System Biosciences SBI

The QuantiMir™ RT Kit is used to generate PCR-ready cDNA from total RNA to measure any small RNA. This system is much more sensitive, quantitative and easier than Northerns or RNase protection experiments. Synthesize enough cDNA template for multiple measurements from a single RT reaction. Profile any small RNA including microRNAs, siRNAs and piRNAs.

• Measure and profile microRNA expression levels from any tissue source
• Validate the existence of predicted and newly discovered microRNA species
• Sensitive down to 10 pg or less of total starting RNA
• A single tube, 3 step process completed in 2 hours
• Rapidly tag and convert all small RNAs into detectable cDNA for thousands of qPCR measurements 

This technology is also available combined with pre-formatted microRNA qPCR assay panels for Cancer microRNAs (OncoMir qPCR Array) and for Stem Cell differentiation-associated (Stem Cell qPCR Array) microRNAs. SBI also offers genome-wide microRNA expression profiling qPCR assay sets.

System Biosciences Cold Fusion Cloning kits use a new and convenient method of DNA cloning. These kits are capable of joining single and multiple PCR fragment(s) into a linearised destination vector in a single step at room temperature.

• PCR product(s) rapidly and accurately fuse into the linearised vector in the desired orientation.
• 5 minute incubation at room temperature followed by 10 minutes on ice.
• Highly efficient system, with more than 95% positive cloning rate.
• Restriction enzyme, ligase and phosphatase free system.
• Competent cells included.
• Supports a broad range of fragment size.

 
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Simultaneously profile 95 different MicroRNAs known to be involved in apoptosis, differentiation and Cancer

• Identify MicroRNA biomarkers and expression pattern signatures
• System rapidly tags and converts all small RNAs into detectable cDNA for qPCRSimple and robust procedure
• Sensitive and quantitative down to picogram amounts of starting total RNA
• Amenable to high-throughput screening of clinical samples including biopsies and LCM samples

Induced pluripotent stem cells can be generated from somatic cells by the transfection of six transcription factors, namely Oct3/4, Sox2, c-Myc, and Klf4, Nanog and Lin-28.  iPS cells are indistinguishable from ES cells in morphology, proliferation gene expression and teratoma formation.

System Biosciences have developed a lentiviral-based protein expression system that can used to reliably deliver these factors to dividing or non-dividing cells. Expressing cells can be monitored or sorted with RFP which is co-expressed. Each of the constructs is available as either plasmid lentivectors or pre-packaged lentivirus

GeneNet™ siRNA Libraries enable you to perform high-throughput gene knockdown studies on a genome-wide basis. You can simultaneously identify multiple genes that alter a specific cellular phenotype—in a single experiment. Click here for more information.

• Screen genome wide for genes regulating cellular responses or signalling pathways
• Find crucial regulatory genes using routine cell selections or flow sorting
• Cost effective genome-wide functional analysis, at prices affordable to the average researcher

Rapid identification of new microRNAs and microRNA like molecules

•  Only 0.5 µg of starting total RNA without size selection
• Amplification and cloning in a single day
• Identify members of two new classes of small non-coding RNAs including the endogenous siRNAs and piRNAs
• Both precursor and mature MicroRNAs can be identified