Lipid nanoparticles and reagent kits: LipidLaunch

Lipid nanoparticle (LNP) exploration and discovery has never been easier with Cayman Chemical's LipidLaunch range. From preformulated and research-ready LNPs, to reagent kits designed to enable LNP uptake visualisation in target cells, Cambridge Bioscience supply Cayman Chemical's LipidLaunch™ range in its entirety to support all levels of LNP research for the advancement of targeted drug and targeted therapy approaches.

Limited time offer: 20% off on your first-time LipidLaunch purchase until 30th June 2024

Now available
Download the Cayman Currents LNP edition issue for a comprehensive guide on the latest LNP technologies and advancements.

Benefits of using Cayman Chemical's LipidLaunch range:
• Research-ready LNPs
• Two LNP formats depending on research needs
• Easy-to-use & adaptable protocols
• Wide variety of applications covered
• From experts in LNP formulation, characterisation & optimisation

► Preloaded LNPs
Cayman's preformulated LNPs containing reporter mRNA are intended for proof-of-concept experiments to help determine whether SM-102-based LNPs can effectively lead to expression of proteins of interest in target cell types (in vitro or in vivo).

Transfection of cells with mCherry-encapsulating SM-102 particles: Huh7 hepatocytes (left) and A549 lung epithelial cells (right) were incubated with LipidLaunch SM-102 (mCherry) at an RNA concentration of 150 ng/ml for 24 hours in cell culture media with 10% FBS. Cells were stained with 4 uM Hoechst 33342 and imaged using 590 LED/Texas Red™ and 365 LED/DAPI cubes on an imaging plate reader.

► Loadable LNPs
Cayman's LipidLaunch Loadable LNP SM-102 Kit provides cargo-ready, lyophilised LNPs while designed to facilitate easy and efficient RNA transfection with reduced toxicity when compared to traditional methods.

Loadable LNPs protocol summary: Following rehydration, loadable LNPs are mixed with RNA cargo before the addition of cell-based assay buffer to promote encapsulation. The loaded LNPs are then ready for downstream delivery to target cells.

► LNP Exploration Kits
Cayman’s LipidLaunch LNP-102 Exploration Kit provides a starting point for researchers to explore the feasibility of using SM-102-based LNPs for specific applications without any requirement for specialised equipment.

Firefly luciferase mRNA encapsulated in LNP-102, LNP-0315 and LNP-MC3 injected subcutaneously into CD-1 IGS mice: In vivo luminescence with intraperitoneal injection of D-luciferin) captured by IVIS 8 hours later.

► LNP Uptake Kits
LNP uptake kits enable evaluation of LNP uptake into your cells of choice, using sterol-based fluorescent tracers. Also compatible with other biological readouts.

LNP-102 uptake in hepatocytes: LNP-102 was formulated using a FRR of 3 and a TFR of 15 ml/min. After dialysis against PBS, LNPs were diluted 1:200 in cell culture media and added to Huh7 hepatocytes for 48 hours. Imaging was carried out in PBS on an imaging plate reader at 20X with a GFP LED/filter cube.

Applications
• Study the LNP transfection efficiency in target cells using reporter gene mRNA-LNPs
• Encapsulate your cargo of interest using preformulated LNPs
• Prepare LNPs without specialised equipment
• Visualise the LNP uptake into your target cell type using fluorescence imaging

Materials available for download
Cayman Currents LNP issue
LipidLaunch SM-102 Preloaded LNP insert
LipidLaunch SM-102 LNP Loadable LNP insert
LipidLaunch LNP-102 Exploration Kit insert
LipidLaunch LNP-102 Uptake Kit insert
LNP efficacy application note