3' mRNA-Sequencing library prep from FFPE samples: QuantSeq FFPE

The QuantSeq FFPE 3’ mRNA-Seq library prep kit from Lexogen enables reliable gene expression profiling even from low-quality samples, utilising protocols and reagents designed to handle and amplify degraded RNA caused by common fixation and storage processes. Optimised for and validated on FFPE samples, while using Lexogen's trusted 3’ mRNA-Seq technology, QuantSeq FFPE minimises bias and provides a more comprehensive view of the gene expression profile. Together with this technology and a streamlined workflow, with clear protocols and fewer manual handling steps, QuantSeq FFPE significantly reduces complexity and time required for library preparation. The kit also includes specific enzyme mixes and reaction conditions designed to combat chemical modifications to RNA within FFPE samples ensuring efficient and consistent library preparation.

Benefits of using QuantSeq FFPE
• Optimised for degraded RNA
• Illumina-compatible UMI-tagged libraries within 4.5 hours
• Detect low abundance transcripts
• No depletion or prior enrichment needed
• Minimise bias with 3’ end sequencing approach
• Significantly reduce preparation time and chance for errors
• Compatible with standard NGS platforms

QuantSeq FFPE provides a streamlined workflow

Applications
Cancer research: Tumor profiling, Retrospective studies
Clinical research: Biomarker discovery, Personalised medicine
Pathology: Molecular diagnostics, Disease mechanism studies 
Pharmaceutical research: Drug development, Toxicology studies 
Translational research: Comparative studies

Materials available for download
QuantSeq FFPE flyer
QuantSeq FFPE user guide
Identifying hallmarks of kidney tumors from FFPE samples poster