seqWell LongPlex: long-read DNA sequencing

Seqwell's LongPlex Multiplexing Kit is an advanced solution tailored to overcome the traditional challenges of long-read sequencing. Designed for researchers requiring reliable, high-quality long-read data, the LongPlex kit addresses common pain points like high sample input needs, sequencing inconsistencies, and lengthy preparation workflows. Leveraging adaptor-loaded transposase DNA fragmentation and indexing, LongPlex enables the efficient multiplexing of up to 96 samples, significantly reducing both time and costs without sacrificing data accuracy.

• Speed: Fragments and tags gDNA using transposase activity, eliminating the need for DNA shearing.
• Simplicity: Plate-based workflow takes less than 2 hours, with only 30 minutes of hands-on effort required.
• Scalability: Easily multiplex samples by pooling up to 96 before proceeding to SMRTbell library preparation.
• Savings: Early pooling reduces the number of SMRTbell library prep rounds required, reducing cost without sacrificing data quality.

LongPlex generates DNA fragments between 8 to 10 kilobases in length, ideal for long-read sequencing.

Benefits of LongPlex

• High multiplexing capability: Efficiently multiplex up to 96 samples per run, cutting down on time and costs.
• Optimised for long-read platforms: Fully compatible with the PacBio Revio, ensuring seamless integration and accurate results. 
• Low sample input requirements: Requires as little as 10 ng of DNA, making it ideal for limited or precious samples.
• Streamlined, user-friendly workflow: A simplified process with just a few hands-on steps, reducing user error and expediting research timelines.
• Cost-effective sequencing: Multiplexing reduces the per-sample cost, making high-quality sequencing accessible to more labs.
• Consistent, high-quality data: Delivers uniform library yields and high read accuracy, minimizing sequencing bias and maximizing data integrity.

Applications of LongPlex

• CRISPR and gene editing validation: Assessing the effectiveness and precision of gene edits through comprehensive sequencing of modified genomes. 
• Long-read genome sequencing: Achieve detailed and accurate genome assemblies with less time and sample input.
• Transcriptome analysis: Capture the full complexity of RNA expression with comprehensive long-read sequencing.
• Epigenetic studies: Analyse methylation and other epigenetic modifications without the need for separate workflows.
• Metagenomics: Uncover the full diversity of complex microbial communities in a single, streamlined workflow.
• Microbiome research: Identify and classify bacteria, fungi, and viruses with high accuracy and resolution.
• Targeted sequencing for variant detection: Focus on specific genomic regions to detect rare or structural variants.
• Structural variant identification: Pinpoint large insertions, deletions, and other structural changes that are challenging to identify with short-read methods.

Material for download

LongPlex user guide

LongPlex product flyer

LongPlex application note - long read microbial whole genome sequencing