Recombinant RFP Antibody

Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, polyclonal and recombinant antibodies for RFP. A single-domain antibody (sdAb) is a small antibody fragment consisting of the monomeric variable domain derived from camelid heavy chain-only immunoglobulins naturally found in llamas, alpacas and camels. Also known as VHH antibodies, these are the smallest functional antigen-binding fragment that occurs in nature (12 - 14 kDa) and are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and potentially therapeutic applications. Anti-RFP is a his-tagged monoclonal recombinant antibody designed to detect Red Fluorescent Protein and its conjugates. This antibody has been tested by dot blot and ELISA and is intended for use in immunological assays including ELISA, immunofluorescence and fluorescence activated cell sorting (FACS) . The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of RFP-conjugated molecules by immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user. This antibody is not suitable for Western blot detection of denatured RFP.