TNKS2 (PARP5B) Colorimetric Assay Kit
The TNKS2 Colorimetric Assay Kit is designed to measure the activity of Tankyrase 2 (TNKS2, also known as PARP5B) for screening and profiling applications. TNKS2 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the TNKS2 Colorimetric Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for TNKS2 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS2 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the Colorimetric HRP substrate to produce color that can be measured using a UV/Vis spectrophotometer microplate reader.
- Data sheet: View or download
- Related Content: PARP assay kits
![image 0](https://www.bioscience.co.uk/product_images/BPS/__686814.78577.png)
![image 1](https://www.bioscience.co.uk/product_images/BPS/__686815.78577_2.png)
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are