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C-CBL-Driven SRC Ubiquitination Intrachain TR-FRET Assay Kit

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384 reactions _$$_
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The C-CBL-driven SRC Ubiquitination Intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin Donor and a Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this assay does not detect mono-ubiquitination. The FRET-based format requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses of polyubiquitination. Figure 1: C-CBL-driven SRC ubiquitination intrachain TR-FRET Assay Kit schematic.

Storage Temperature:

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are

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