E. coli in vivo Biotinylation Kit (with BirA Competent Cells)

The E. coli in vivo biotinylation kit comes in a convenient, easy-to-use format and contains all the components necessary to label biotin-acceptor peptides with biotin in E. coli. Strain BL21, a chemically competent E. coli B strain. The cells include an IPTG-inducible plasmid containing the BirA gene and the resistance gene for streptomycin or spectinomycin. Just transform the competent cells with a vector expressing your protein of interest with an AviTag™ sequence (example: pAN or pAC vectors). After transformation, BirA expression is induced and biotin is added to the culture. The BirA efficiently catalyzes the specific attachment of biotin to the AviTag. This product is for research use only and is subject to a Limited Use License. For your order to be processed, you will be required to complete a Limited Use License Agreement form at: https://bpsbioscience.com/license/agreement/form, ensuring you state your Purchase Order number when asked for Sales Order/Quote #. Further information can be found at: https://bpsbioscience.com/license.

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