Purity:
≥95% by HPLC.
Molecular Weight:
8722
Product Description:
Ubiquitin-AMC is a fluorogenic substrate for ubiquitin hydrolases based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Upon incubation with a protease recognizing Ubiquitin, such as USP2 or UCHL3, AMC is released and the increase in fluorescence can be measured using Ex380 nm and Em460 nm wavelengths. This protein contains no extraneous tags.
Storage Temperature:
Store in the dark at or below -80°C. Stable as supplied for up to 1 year when stored dessicated at -