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anti-Cas9 (S. aureus) antibody

Catalogue number:
ARG10681-50ul
Size:
50 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£394.00 Shipping is calculated in checkout
Antibody Host:

Chicken

Target:

Cas9 (S. aureus)

Antibody Isotype:

IgY

Antibody Type:

Polyclonal

image 0
image 1
Product Description:

anti-Cas9 (S. aureus) antibody - 50 µl

Storage Temperature:

-20°C

Immunocytochemistry: Transfected HEK293
Immunocytochemistry: Transfected HEK293 cells which overexpressing GFP-Cas9 (SA) fusion protein were stained with ARG10681 anti-Cas9 (S. aureus) antibody. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining with ARG10681 anti-Cas9 (S. aureus) antibody is shown in red in middle panel. In merged image (right panel), most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.
Western blot: 1) HEK293 cells overexpres
Western blot: 1) HEK293 cells overexpressing a fusion protein containing GFP and the C-terminus of Cas9 from S. aureus, and 2) Non-transfected HEK293 cells stained with ARG10681 anti-Cas9 (S. aureus) antibody at 1:1000 dilution.
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