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anti-Aldolase C antibody [4A9]

Catalogue number:
ARG10695-50ul
Size:
50 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£394.00 Shipping is calculated in checkout
Antibody Host:

Mouse

Target:

Aldolase C

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

anti-Aldolase C antibody [4A9] - 50 µl

Storage Temperature:

-20°C

Immunocytochemistry: Rat mixed neuron /
Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10695 anti-Aldolase C antibody [4A9] (green) and co-stained with our rabbit antibody to FOX3 / NeuN (red). ARG10695 anti-Aldolase C antibody [4A9] antibody reveals strong cytoplasmic staining in astrocytes, while Rabbit FOX3 / NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and is complete absence of astrocytes. Blue is a DNA stain.
Immunofluorescence: Cortical neuron-glia
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10695 anti-Aldolase C antibody [4A9] (green) at 1:1000 dilution and costained with ARG52328 anti-MAP2 antibody (red) at 1:10000 dilution. Hoechst (blue) for nuclear staining.
Immunohistochemistry: Frozen section of
Immunohistochemistry: Frozen section of Rat cerebellum stained with ARG10695 anti-Aldolase C antibody [4A9] (green) at 1:1000 dilution and costained with ARG52312 anti-GFAP antibody (red) at 1:5000 dilution. Hoechst (blue) for nuclear staining.
Immunohistochemistry: Frozen sections of
Immunohistochemistry: Frozen sections of Mouse brain (fixed by transcardial perfusion with 4% paraformaldehyde) stained with ARG10695 anti-Aldolase C antibody [4A9] (red) and co-stained with chicken anti-Vimentin  antibody (green). In the striatum, ARG10695 anti-Aldolase C antibody [4A9] positive astrocytes are highly co-stained with Vimentin  antibody, which results in yellow to gold colors. Insets show a higher magnification picture. Nuclei are labeled with DAPI (blue).
Immunohistochemistry: Frozen section of
Immunohistochemistry: Frozen section of E20 Rat cortical tissue stained with ARG10695 anti-Aldolase C antibody [4A9] (green) at 1:1000 dilution and costained with ARG52328 anti-MAP2 antibody (red) at 1:10000 dilution.
Immunohistochemistry: Frozen sections of
Immunohistochemistry: Frozen sections of Mouse brain (fixed by transcardial perfusion with 4% paraformaldehyde) stained with ARG10695 anti-Aldolase C antibody [4A9] (red) and co-stained with chicken anti-Vimentin  antibody (green). In the cerebellum, ARG10695 anti-Aldolase C antibody [4A9] positive Purkinje cells do not express vimentin, which results in red color. Insets show a higher magnification picture. Nuclei are labeled with DAPI (blue).
Western blot: Crude cow cerebellum homog
Western blot: Crude cow cerebellum homogenate stained with ARG10695 anti-Aldolase C antibody [4A9]. Other studies show that this antibody does not recognize the very closely related molecules aldolase A or B.
Western blot: Rat brain, Mouse brain, Co
Western blot: Rat brain, Mouse brain, Cow cerebellum and Pig hippocampus lysates stained with ARG10695 anti-Aldolase C antibody [4A9] (green) at 1:2000 dilution.
Western blot: Recombinant Human Aldolase
Western blot: Recombinant Human Aldolase A, B and C with three monoclonal antibodies as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged Human Aldolase A, B and C respectively. The epitope for ARG10695 anti-Aldolase C antibody [4A9] is within the N-terminal peptide of the molecule which is less conserved between the three Aldolases. Clone 1A1 binds to the C-terminal peptide and also is specific for Aldolase C, while clone E9 binds to the less conserved core of the molecule and recognizes all three Aldolase C gene products.
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