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anti-Cas9 (S. pyogenes) antibody [3F9]

Catalogue number:
ARG10706-50ul
Size:
50 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£394.00 Shipping is calculated in checkout
Antibody Host:

Mouse

Target:

Cas9 (S. pyogenes)

Antibody Isotype:

IgG

Antibody Type:

Monoclonal

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Product Description:

anti-Cas9 (S. pyogenes) antibody [3F9] - 50 µl

Storage Temperature:

-20°C

Immunocytochemistry: Transfected HEK293
Immunocytochemistry: Transfected HEK293 cells overexpressing the N-terminal aa. 1-608 of S. pyogenes Cas9. The cells were stained with ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] (red), and these cells also co-stained with ARG10734 anti-Cas9 antibody to the same construct in green, giving a yellow color. The N-terminal construct contains a nuclear localization sequence and so is predominantly nuclear in localization. Most HEK293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain.
Western blot: 1) MW marker, 2) crude pro
Western blot: 1) MW marker, 2) crude protein extract from Hek293 culture transfected with the immunogen, the N-terminal 1-608 amino acids, 3) Non-transfected control Hek293 cell extract, and 4) 40 ng of Streptococcus pyogenes Cas9 (full length) was stained with ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] at 1:1000 dilution.
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