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anti-MeCP2 antibody [5H12]

Catalogue number:
ARG10750-50ul
Size:
50 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£394.00 Shipping is calculated in checkout
Antibody Host:

Mouse

Target:

MeCP2

Antibody Isotype:

IgG2b

Antibody Type:

Monoclonal

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Product Description:

anti-MeCP2 antibody [5H12] - 50 µl

Storage Temperature:

-20°C

Immunohistochemistry: Frozen section of
Immunohistochemistry: Frozen section of Rat cerebellum stained with ARG10750 anti-MeCP2 antibody [5H12] (red) at 1:1000 dilution and costained with ARG52312 anti-GFAP antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 µM, and free-floating sections were stained with the above antibodies.)
Immunohistochemistry: Frozen sections of
Immunohistochemistry: Frozen sections of Rat hippocampus fixed by transcardial perfusion with 4% paraformaldehyde, cut to 45 µM and stained with ARG10750 anti-MeCP2 antibody [5H12] (red). Staining for NeuN / Fox3 using our rabbit polyclonal antibody (green). The Fox3 / NeuN antibody stains neuronal nuclei and proximal cytoplasm in the granular layer while MeCP2 staining is only nuclear and, interestingly, is much stronger in certain cells. Blue shows DAPI staining of nuclear DNA.
Western blot: A nuclear extract from mou
Western blot: A nuclear extract from mouse brain with ARG10750 anti-MeCP2 antibody [5H12]. This antibody recognizes a strong and clear band at 74 kDa corresponding to full length MeCP2. The calculated molecular weight of full length MeCP2 is 54 kDa, but the molecule runs on SDS-PAGE gels at 74 kDa due to the highly charged and very basic properties of the molecule.
Western blot: Rat brain (nuclear), Mouse
Western blot: Rat brain (nuclear), Mouse brain (nuclear) and Cow cerebellum lysates stained with ARG10750 anti-MeCP2 antibody [5H12] (green) at 1:2000 dilution.
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