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  3. anti-Acrolein antibody [10A10]
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anti-Acrolein antibody [10A10]

Catalogue number:
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ARG22620-50ug
Supplier:
Arigo Biolaboratories
Size:
50 µg _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
£449.00
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Antibody Host:

Mouse

Target:

Acrolein

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

anti-Acrolein antibody [10A10] - 50 µg

Storage Temperature:

-20°C

Immunofluorescence: 5% Formaldehyde fixe
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22620 anti-Acrolein antibody [10A10] at 1:50 for 30 - 60 min at RT. Secondary Antibody: Goat anti-Mouse (Alexa Fluor 488) at 1:1500 for 30 - 60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain. (A,C,E,G) Untreated. (B,D,F,H) Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibody. (G,H) Composite.
Flow Cytometry: 90% Methanol fixed Neuro
Flow Cytometry: 90% Methanol fixed Neuroblastoma cells (SH-SY5Y) stained with ARG22620 anti-Acrolein antibody [10A10] at 1:50 for 30 min on ice. Secondary Antibody: Goat anti-Mouse (PE) at 1:100 for 20 min at RT. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours. Dashed line: Non specific IgG control. Solid line: Primary antibodies.
Western blot: Acrolein-BSA (0.5 µg), Acr
Western blot: Acrolein-BSA (0.5 µg), Acrolein-BSA (2.0 µg), BSA (0.5 µg), and BSA (2.0 µg) stained with ARG22620 anti-Acrolein antibody [10A10] at 1:1000 for 2 hours at RT.
Western blot: 12 µg of HeLa cell lysate
Western blot: 12 µg of HeLa cell lysate and H2O2 treated HeLa cell lysate stained with ARG22620 anti-Acrolein antibody [10A10] at 1:1000 for 2 hours at RT.
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