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anti-GFP antibody

Catalogue number:
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ARG62993-100ug
Supplier:
Arigo Biolaboratories
Size:
100 µg _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
£473.00
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Antibody Host:

Rabbit

Target:

GFP

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

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image 1
Product Description:

anti-GFP antibody - 100 µg

Storage Temperature:

-20°C

Immunofluorescence: Confocal microscopy
Immunofluorescence: Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP; bottom).The natural fluorescence of the produced proteins is shown in the green channel (left), ARG62993 anti-GFP antibody signal was detected in the red channel (right). The blue nuclear stain is also shown.
Immunoprecipitation of GFP-NLS from HEK2
Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. 20 hours post transfection cells were lysed in non-denaturating conditions (Lysis buffer: 20 mM Tris, pH 7.5, 100 mM NaCl, 0.5% Triton X-100, inhibitors of proteases). Aliquots of cell lysate were immunoprecipitated with ARG62993 anti-GFP antibody (lane 2) or a pre-immune rabbit serum (lane 3). Immunoprecipitates together with a sample of the cell lysate (lane 1) were separated on SDS-PAGE polyacrylamide gel and stained with ARG62993 anti-GFP antibody.
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