HOT FIREPol® Multiplex qPCR Mix (ROX)

Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction.Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types Analyze 1-4 targets in 1 reaction High specificity and sensitivity Robust amplification of GC-rich targets Contains dUTP to prevent cross-contamination when used in combination with UNGHOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2 and Purple passive reference dye according to system requirements.HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity.HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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