SOLIScript® 1-step CoV Kit

Sensitive and highly specific 1-step RT-qPCR reagents optimized for SARS-CoV-2 detection.Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip® RNase inhibitor, and nuclease-free water. Optimized for SARS-CoV-2 detection Flexible assay set-up Suitable for multiplexing (up to 4 targets in a single assay) Reaction set-up and shipment without ice RNase inhibitor included in the kitSOLIScript® 1-step CoV Kit is optimized for highly sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA by one-step real-time RT-PCR (RT-qPCR).The Kit is suitable for the detection of up to four targets simultaneously and can be used with all common qPCR cyclers. Follow recommendations specific for a selected SARS-CoV-2 diagnostic panel, and instrument-specific guidelines. Contact us if a passive reference dye is required for signal normalization.SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user. If full solution is needed, please check out: SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit.SOLIScript® 1-step CoV Kit is tested and validated for SARS-CoV-2 detection by diagnostic laboratories. Please see our recommendations for US-CDC and Berlin-Charité protocols. Note! Amplification of more than one target in a single reaction may require considerable optimization, individual assays must be validated prior to multiplexing. For multiplex reactions, design primer pairs with similar annealing temperatures for all targets to be amplified. Self-complementarity of primers should be avoided.

• Data sheet: View or download
• Protocol: View or download