sPLA2 (Type IIA) Inhibitor Screening Assay Kit
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giic spla2, group iia phospholipase a2, non-pancreatic secretory phospholipase a2, phosphatidylcholine 2-acylhydrolase 2a, phospholipase a2, membrane associated, pla2g2a, secretory phospholipase a2 (group iia), spla2-iia
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- MSDS: View or download
Cayman’s sPLA2 (Type IIA) Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human sPLA2 type IIA, a phospholipase that preferentially hydrolyzes PG and PE. The assay uses diheptanoyl thio-PE, a sulfur-containing analog of PE as a substrate. Upon hydrolysis by sPLA2 type IIA at the sn-2 position, the free thiol can be detected by the sPLA2 Assay Probe. This results in a product that can be easily quantified by measuring absorbance at a wavelength of 410 nm. A potent and reversible inhibitor, LY315920 (included in this kit as sPLA2 Control Inhibitor), inhibits the enzyme with an average IC50 value of approximately 22 nM.
-80°C