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  3. A700-029-T
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Rabbit anti-CD86 Recombinant Monoclonal Antibody [BLR029F]

Catalogue number:
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A700-029-T
Supplier:
Bethyl Laboratories, Inc.
Size:
10 µl (5+ tests) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£102.40
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Estimated delivery Wed 24 Jul
Special price until 31 Jul 2024
Normal price £128.00
Shipping is calculated in checkout
Applications:

WB, IP, ICC, Flow Cyt

Antibody Host:

Rabbit

Target:

CD86

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

Residues 31-245 (ECD)

Alternative Names:

CD28LG2; Activation B7-2 antigen; LAB72; T-lymphocyte activation antigen CD86; FUN-1; CTLA-4 counter-receptor B7.2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); BU63; B-lymphocyte activation antigen B7-2; B7-2; B7.2; CD antigen CD86; B70

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Product Description:

Rabbit anti-CD86 Recombinant Monoclonal Antibody [BLR029F] - 10 µl (5+ tests)

Storage Temperature:

2 - 8°C

Detection of human CD86 by immunocytoche
Detection of human CD86 by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody [BLR029F] (A700-029-T lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human CD86 by western blot.
Detection of human CD86 by western blot. Samples: Whole cell lysate (5 and 50 µg) from HDLM-2, MJ, SUP-M2, KG-1, MOLT-4, Ramos, Jurkat, HeLa, HEK293T, and RAW 264.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody [BLR029F] (A700-029-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).
Detection of human CD86 by western blot
Detection of human CD86 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HDML-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD86 recombinant monoclonal antibody [BLR029F] (A700-029-T lot 1) used for IP at 6 µl per reaction. CD86 was also immunoprecipitated by rabbit anti-CD86 recombinant monoclonal antibody [BLR030F] (A700-030). For blotting immunoprecipitated CD86, A700-029-T was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
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