CD200R3, Mouse, mAb Ba103

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The monoclonal antibody Ba103 recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38kDa or ~74 kDa (reduced or non-reduced condition, respectively). CD200R3 presumably exists as adisulfide-linked dimer on the cell surface, it is a single-pass type I membrane protein.The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R containstwo Ig-like extracellular domains, one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type(immunoglobulin-like) domain. and mediates inhibitory signals in myeloid cells. Mice deficient for theligand CD200 develop enhanced experimental allergic encephalomyelitis and collagen-induced arthritis.Unlike CD200R, the CD200R-like receptors function as activating receptors. They contain shortcytoplasmic tails and a lysine residue in the transmembrane region and are likely to signal via adaptorproteins such as DAP12, DAP10, FcRg, or CD3z.CD200R3 is expressed in uterus and bone marrow-derived mast cells (at protein level). It is expressed inuterus, spleen, bone marrow-derived dendritic, basophil and mast cells. CD200R3 is also expressed inthe lung of N. brasiliensis-infected mice. It is weakly expressed in brain, testis, lung and thymus.CD200R3 is expressed preferentially on cells of the myeloid lineage, including mast cells and basophils.Both cell types are involved in the host defense system against pathogens and in the development ofallergic disorders. They function as independent essential initiators of allergic reactions. Basophils are theleast common leukocytes in the peripheral blood accounting approximately 0.5 % of all leukocytes. In vivodepletion of basophils by using monoclonal antibody Ba103 proved the essential role of basophils in thedevelopment and maintenance of IgE-mediated chronic allergic inflammation.The activating CD200 receptors, like CD200R3, play an important role in IgE-independent mast cell andbasophil activation. Monoclonal antibody Ba103 activates basophils ex vivo and elicits systemicanaphylaxis when administered in vivo. Cross-linking of CD200R3 on MC/9 cells by monoclonal antibodyBa103 induces degranulation. Furthermore, monoclonal antibody Ba103 is useful for depletion ofbasophils.