HOT FIREPol® EvaGreen® HRM Mix (no ROX)

Optimized ready-to-use solution for High Resolution Melt (HRM) Analysis. Highly sensitive mix for accurate SNP detection Sensitive EvaGreen® dye for reliable HRM performance Reaction set-up without dry iceHigh-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations.5x HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for HRM analysis, incorporating EvaGreen® dye.It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen® dye. The user simply needs to add water, template, and primers.HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers.

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