HOT FIREPol® EvaGreen® qPCR Supermix

Optimized ready-to-use solution for dye-based real-time quantitative PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions. High sensitivity with low DNA concentrations Blue dye to ease pipetting Reduced primer dimer formationone qPCR mix for all cyclers (except capillary and high ROX cyclers) Contains dUTP to prevent carryover Contamination with UNG treatmentHOT FIREPol® EvaGreen® qPCR Supermix is an optimized 5X reaction mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments.The Mix contains HOT FIREPol® DNA Polymerase and a passive internal reference dye that is compatible with most qPCR cyclers including no ROX and low ROX reference signal requiring platforms. The Mix contains dUTPs to prevent cross-contamination with UNG treatment and visible dye to ease reaction set-up. This dye does not interfere with real-time detection.

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