HOT FIREPol® SolisGreen® qPCR Mix

Highly sensitive dye-based qPCR Mix with improved detection of low-copy targets.Optimized ready-to-use solution for dye-based real-time quantitativePCR assays. SolisGreen® dye is characterized by high sensitivity andgreat PCR efficiency with low template amounts for accurate andreproducible results Improved detection of low target concentrations Higher fluorescence level Reaction set-up and shipment without dry iceDye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye.It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template, and primers.HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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