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  3. anti-Ubiquilin 2 antibody [6H9]
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anti-Ubiquilin 2 antibody [6H9]

Catalogue number:
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ARG10728-50ul
Supplier:
Arigo Biolaboratories
Size:
50 µl _$$_
Product is available in:
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£394.00
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Antibody Host:

Mouse

Target:

Ubiquilin 2

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

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Product Description:

anti-Ubiquilin 2 antibody [6H9] - 50 µl

Storage Temperature:

-20°C

Immunofluorescence: NIH/3T3 cells staine
Immunofluorescence: NIH/3T3 cells stained with ARG10728 anti-Ubiquilin 2 antibody [6H9] (green) at 1:1000 dilution and costained with ARG10685 anti-Lamin A + C antibody (red) at 1:5000 dilution. DAPI (blue) for nuclear staining.
Immunocytochemistry: HeLa cell cultures
Immunocytochemistry: HeLa cell cultures stained with ARG10728 anti-Ubiquilin 2 antibody [6H9] (green) and co-stained with chicken polyclonal antibody to vimentin (red). In most individual cells, ubiquilin 2 is present diffusely in the cytoplasm of cells, though some cells show enrichment of the protein in spherical autophagosome-like structure.
Western blot: Ubiquilin 1 and Ubiquilin
Western blot: Ubiquilin 1 and Ubiquilin 2 recombinant stained with ARG10728 anti-Ubiquilin 2 antibody [6H9].
Western blot: 1) Untransfected primary M
Western blot: 1) Untransfected primary Mouse neuron and glia cell cultures, 2) the same cells transduced with Human ubiquilin 2 wild type, 3) with ubiquilin 2 P506T mutant, 4) with ubiquilin 2 P497S mutant, and 5) with enhanced GFP control; 6) HeLa cells, and 7) 3T3 cells stained with ARG10728 anti-Ubiquilin 2 antibody [6H9]. In primary mouse neuron and glia cell culture, endogenous ubiquilin 2 appears as a weak band at 68 kDa in all transduced and non-transduced cells, indicating low endogenous expression of mouse ubiquilin 2. Strong bands are seen in the cells transduced with Human wild type or mutant ubiquilin 2. Small proteins run at 50 kDa in these cells are the fragments of ubiquilin 2. Note, ubiquilin 2 run at ~66 kDa in Human HeLa cells and 68 kDa in rodent 3T3 cells.
Western blot: NIH/3T3, C6, HEK293, HeLa,
Western blot: NIH/3T3, C6, HEK293, HeLa, SH-SY5Y, Rat whole brain and Mouse whole brain lysates stained with ARG10728 anti-Ubiquilin 2 antibody [6H9] (green) at 1:1000 dilution.
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