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anti-Neurofilament NF-L antibody

Catalogue number:
ARG10732-50ul
Size:
50 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£394.00 Shipping is calculated in checkout
Antibody Host:

Rabbit

Target:

Neurofilament NF-L

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

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Product Description:

anti-Neurofilament NF-L antibody - 50 µl

Storage Temperature:

-20°C

Immunocytochemistry: Mixed neuron / glia
Immunocytochemistry: Mixed neuron / glia cultures from newborn Rat brain stained with monoclonal 7C5 to peripherin (green) and co-stained with ARG10732 anti-Neurofilament NF-L antibody (red). A class of large neurons, like the one in the middle of this image, contains peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the peripherin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this image. The blue channel reveals the localization of DNA.
Immunohistochemistry: Frozen section of
Immunohistochemistry: Frozen section of Mouse cerebellum stained with ARG10732 anti-Neurofilament NF-L antibody (red) at 1:5000 dilution and costained with ARG52344 anti-Myelin Basic Protein antibody (green) at 1:5000 dilution. (Sample preparation: Following transcardial perfusion of Mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)
Western blot: Whole Rat brain homogenate
Western blot: Whole Rat brain homogenate stained with ARG10732 anti-Neurofilament NF-L antibody at 1:15000 dilution. A prominent band running with an apparent SDS-PAGE molecular weight of ~68 kDa corresponds to rodent NF-L.
Western blot: Rat brain, Rat spinal cord
Western blot: Rat brain, Rat spinal cord, Mouse brain and Mouse spinal cord lysates stained with ARG10732 anti-Neurofilament NF-L antibody (green) at 1:20000 dilution.
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