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  3. anti-Cas9 (S. Pyogenes) antibody
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anti-Cas9 (S. Pyogenes) antibody

Catalogue number:
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ARG10734-50ul
Supplier:
Arigo Biolaboratories
Size:
50 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
£394.00
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Antibody Host:

Rabbit

Target:

Cas9 (S. Pyogenes)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

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Product Description:

anti-Cas9 (S. Pyogenes) antibody - 50 µl

Storage Temperature:

-20°C

Immunocytochemistry: Hek293 cell culture
Immunocytochemistry: Hek293 cell culture transfected with a construct including the C-terminal 814-1372 amino acids of Staphylococcus  pyogenes Cas9. The cells were stained with ARG10734 anti-Cas9 (S. Pyogenes) antibody (red) and also with a chicken antibody to S. pyogenes Cas9 (green). A cell expressing Cas9 therefore appears yellow. Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with the blue DNA stain.
Western blot: 1) 100 ng, and 2) 10 ng of
Western blot: 1) 100 ng, and 2) 10 ng of full length Cas9 protein from Streptococcus pyogenes was stained with ARG10734 anti-Cas9 (S. Pyogenes) antibody at 1:2000 dilution. This antibody recognizes full length Cas9 protein at 160 kDa and a proteolytic fragment at 130 kDa.
Western blot: 1) HEK293 (non-transfected
Western blot: 1) HEK293 (non-transfected), 2) transfected HEK293 expressing the C-terminal aa. 814-1372 of Cas9 from S. pyogenes, 3) transfected HEK293 expressing the N-terminal 608 amino acids of Cas9 from S. pyogenes and 4) 40 ng of full length S. pyogenes Cas9 protein. The blots were stained with ARG10734 anti-Cas9 (S. Pyogenes) antibody (green) at 1: 1000 dilution.
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