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anti-c-Fos antibody

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ARG10733-50ul
Supplier:
Arigo Biolaboratories
Size:
50 µl _$$_
Product is available in:
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£394.00
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Antibody Host:

Rabbit

Target:

c-Fos

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

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Product Description:

anti-c-Fos antibody - 50 µl

Storage Temperature:

-20°C

Western blot: 1) HeLa (FBS free), 2) HeL
Western blot: 1) HeLa (FBS free), 2) HeLa treated with 20% FBS for 2 hours after being in FBS free media for 36 hours, 3) Rat cortical neurons and 4) Rat cortical neurons treated with membrane depolarization buffer for 5 hours. The blots were stained with ARG10733 anti-c-Fos antibody (green) at 1:5000 dilution and ARG52320 anti-GAPDH antibody [1D4] (red) at 1:5000 dilution.
Immunocytochemistry: ARG10733 anti-c-Fos
Immunocytochemistry: ARG10733 anti-c-Fos antibody staining (red) in HeLa cells which were treated with serum-starvation for 36 hours, followed by 2 hours, 20% FBS stimulation (bottom panel), or followed by PBS treatment (top panel). Red c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells were co-stained with a chicken polyclonal antibody against vimentin (green). Blue shows DAPI staining of nuclear DNA.
Immunofluorescence: HeLa cells stained w
Immunofluorescence: HeLa cells stained with ARG10733 anti-c-Fos antibody (red) at 1:5000 dilution and Mouse mAb to tubulin (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
Immunofluorescence: Mouse hippocampus se
Immunofluorescence: Mouse hippocampus sections stained with ARG10733 anti-c-Fos antibody (red) at 1:20000 dilution and Mouse mAb to NF-L (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. Following transcardial perfusion of Mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.
Immunofluorescence: Mouse olfactory bulb
Immunofluorescence: Mouse olfactory bulb sections stained with ARG10733 anti-c-Fos antibody (red) at 1:20000 dilution and Mouse mAb to NF-L (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. Following transcardial perfusion of Mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.
Immunohistochemistry: Mouse brain sectio
Immunohistochemistry: Mouse brain section (45 µM; fixed by transcardial perfusion with 4% paraformaldehyde) stained with ARG10733 anti-c-Fos antibody using a standard HRP-DAB staining technique. Cells expressing c-Fos appear to be dark.
Immunohistochemistry: Frozen sections of
Immunohistochemistry: Frozen sections of Mouse brain across hippocampus stained with ARG10733 anti-c-Fos antibody (red) and co-stained with anti-Fox3 / NeuN antibody (green) using immuno-fluorescent microscopy. Neurons positive for c-Fos and Fox3 / NeuN appear to be yellow. Inset shows an enlarged image. Nuclei are stained with DAPI (blue).
Western blot: Top panel: 1) HeLa cells w
Western blot: Top panel: 1) HeLa cells were serum-starved for 36 hours, and 2) Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. ARG10733 anti-c-Fos antibody recognizes bands with apparent molecular weight of 50-65 kDa, representing multiple forms of the c-Fos protein. These bands appear in serum stimulated cells and are absent in serum-starved HeLa cells. Bottom panel:  Blot was stripped and stained with a monoclonal antibody (Clone: 1D4) against GAPDH, used as loading control.
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