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anti-MeCP2 antibody

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ARG10737-50ul
Supplier:
Arigo Biolaboratories
Size:
50 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
£394.00
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Antibody Host:

Rabbit

Target:

MeCP2

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

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Product Description:

anti-MeCP2 antibody - 50 µl

Storage Temperature:

-20°C

Immunofluorescence: COS-1 cells stained
Immunofluorescence: COS-1 cells stained with ARG10737 anti-MeCP2 antibody (red) at 1:5000 dilution and costained with Mouse mAb to ALDHL1 (green) at 1:1000 dilution. DAPI (blue) for nuclear staining. Following fixation with 3.7% formalin for 5 min, cells were permeabilized with ice cold methanol, blocked and stained with above antibodies.
Immunohistochemistry: Frozen section of
Immunohistochemistry: Frozen section of Rat cerebellum stained with ARG10737 anti-MeCP2 antibody (red) at 1:5000 dilution and costained with Chicken pAb to calbindin (green) at 1:2000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)
Immunohistochemistry: Frozen sections of
Immunohistochemistry: Frozen sections of Mouse brain, which was cut at 45 µm on a vibratome after perfusion with 4% paraformaldehyde and overnight fixation, was stained with ARG10737 anti-MeCP2 antibody. MeCP2 was mainly associated with  methylated DNA in nucleus of neuron cells  (green). Blue shows DAPI staining of nuclear DNA.
Western blot: A nuclear extract from mou
Western blot: A nuclear extract from mouse brain with ARG10737 anti-MeCP2 antibody. This antibody recognizes a strong and clear band at 75 kDa corresponding to total MeCP2  of mouse brain in SDS-PAGE, while molecular weight of MeCP2 protein is 54 kDa.
Western blot: Rat brain (whole), Rat bra
Western blot: Rat brain (whole), Rat brain (nuclear), Mouse brain (whole), Mouse brain (nuclear), HeLa and C6 cell lysates stained with ARG10737 anti-MeCP2 antibody (green) at 1:20000 dilution.
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