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anti-mCherry antibody

Catalogue number:
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ARG10758-50ul
Supplier:
Arigo Biolaboratories
Size:
50 µl _$$_
Product is available in:
  • UK
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£394.00
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Antibody Host:

Chicken

Target:

mCherry

Antibody Isotype:

IgY

Antibody Type:

Polyclonal

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Product Description:

anti-mCherry antibody - 50 µl

Storage Temperature:

-20°C

Immunofluorescence: HEK293 cells transfe
Immunofluorescence: HEK293 cells transfected with a mCherry (red). Cells were stained with ARG10758 anti-mCherry antibody (green). DNA staining (blue).
Western blot: 1) HEK293 (non-transfected
Western blot: 1) HEK293 (non-transfected), 2) HEK293 transfected with tdTomato construct, 3) HEK293 transfected with mCherry-HA construct, and 4) HEK293 transfected with GFP construct. The blots were stained with ARG10758 anti-mCherry antibody (green) at 1:2000 dilution. The same blot was simultaneously stained with Mouse mAb to GAPDH (red) at 1:2000 dilution.
Immunocytochemistry: HEK293 cells transf
Immunocytochemistry: HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with ARG10758 anti-mCherry antibody is shown in Green. Green antibody staining is only seen cells which express mCherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight mCherry fluorochrome.
Western blot: 1) HEK293 cells transfecte
Western blot: 1) HEK293 cells transfected with pFin-EF1-mCherry vector, and 2) HEK293 cells which were not transfected with this vector. The blots were stained with ARG10758 anti-mCherry antibody at 1: 2000 dilution.
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