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anti-mCherry antibody

Catalogue number:
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ARG10760-50ul
Supplier:
Arigo Biolaboratories
Size:
50 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
£394.00
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Antibody Host:

Rabbit

Target:

mCherry

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

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Product Description:

anti-mCherry antibody - 50 µl

Storage Temperature:

-20°C

Immunocytochemistry: HEK293 cells transf
Immunocytochemistry: HEK293 cells transfected in the same way and viewed using a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with ARG10760 anti-mCherry antibody is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome.
Western blot: HEK293 cells transfected w
Western blot: HEK293 cells transfected with pFin-EF1-mCherry vector ("+"), and HEK293 cells which were not transfected with this vector ("-"). The blots were stained with ARG10760 anti-mCherry antibody.
Western blot: 1) HEK293 (non-transfected
Western blot: 1) HEK293 (non-transfected), 2) HEK293 transfected with mCherry-HA construct, 3) Recombinant mCherry protein, 4) Recombinant GFP protein and 5) HEK293 transfected with a full length GFP construct. The blots were stained with ARG10760 anti-mCherry antibody (green) at 1:1000 dilution. The same blot was simultaneously stained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution.
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