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  3. anti-mCherry antibody [1C51]
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anti-mCherry antibody [1C51]

Catalogue number:
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ARG10759-50ul
Supplier:
Arigo Biolaboratories
Size:
50 µl _$$_
Product is available in:
  • UK
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£394.00
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Antibody Host:

Mouse

Target:

mCherry

Antibody Isotype:

IgG2a

Antibody Type:

Monoclonal

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Product Description:

anti-mCherry antibody [1C51] - 50 µl

Storage Temperature:

-20°C

Immunocytochemistry: HEK293 cells transf
Immunocytochemistry: HEK293 cells transfected with mCherry and visualized in red. The cells were stained with ARG10759 anti-mCherry antibody [1C51] in green, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the ARG10759 anti-mCherry antibody [1C51]. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue).
Western blot: Crude extract of HEK293 ce
Western blot: Crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector in lane labelled "+". The "-" lane is a blot of an equal amount of protein extract from untransfected HEK293 cells. ARG10759 anti-mCherry antibody [1C51] binds a major band running at ~28 kDa corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells.
Western blot: 1) HEK293 (non-transfected
Western blot: 1) HEK293 (non-transfected), 2) HEK293 transfected with mCherry-HA construct, 3) Recombinant mCherry protein, 4) Recombinant GFP protein and 5) HEK293 transfected with GFP construct. The blots were stained with ARG10759 anti-mCherry antibody [1C51] (green) at 1:1000 dilution. The same blot was simultaneously stained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution.
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